Charlene "Charley" Davidson (voiced by Leeza Miller-McGee) is a human mechanic, who owns the Last Chance Garage in Chicago. She is a headstrong woman and is always ready to go into battle, though the Biker Mice try to keep her out of dangerous situations, never because they think she's incapable or weak, but because they couldn't bear anything bad happening to her. Aside from being the target of Vinnie's affection (which she playfully blows off), she is also responsible for repairs and upgrades to the Mice's bikes, is a high-tech genius in her own right, and is as skilled a biker as the Mice. Her name is an obvious pun on Harley Davidson.
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